Ocean Optics Ocean View Install - Manual (Page 59)

4: Wizards
000-20000-310-02-201312 51
►
Procedure
1. Invoke the Photometry wizard by clicking the Create new spectroscopy application icon .
2. Select Photometry.
3. Use the Photometry Choice panel to select the absolute irradiance process used to calculate the
photometric data.
d. If an absolute irradiance wizard has already been run, select it under Existing processing.
--OR--
e. If no absolute irradiance process already exists, select New absolute irradiance processing
to begin. You will complete the steps for the Absolute Irradiance before being brought
back to complete the photometry wizard.
4. Use the Photometry Parameters to set the Observer Luminosity Function and the solid angle of
the source. The Observer Luminosity Function is displayed in the preview graph.
Concentration Wizard
Concentration is the amount of a specified substance in a solution. Graphs of absorbance vs.
concentration are known as Beer’s Law plots. These are prepared by measuring the light absorbed by a
series of solutions with different known concentrations.
The length of the sample, such as the path length of our cuvette holder, and the wavelength chosen for
monitoring the amount of light absorbed are constants. A linear plot from taking scans of these standard
solutions with known concentrations is then obtained. The plot is then used to determine the unknown
concentrations of substances in solutions.
►
Procedure
1. Invoke the Concentration wizard by clicking the Create new spectroscopy application icon .
2. Select Concentration.
3. Use the Concentration Choice panel to choose between two different concentration models:
a. Beer-Lambert Law -- Use this model when you know the Molar Extinction Coefficient
( ) and Path Length (l) of the unknown solution.
b. Calibrate from solutions of known concentration using a series of known solutions
4. Use the Existing Absorbance panel to select the absorbance process used to calculate the
concentration data.
c. If no absorbance process already exists, select New to begin. You will complete the steps
for the Absorbance wizard before being brought back to complete the Concentration
wizard.
d. If an Absorbance wizard has already been run, select it under Select existing
Absorbance process.