Ocean Optics Ocean View Install - Manual (Page 53)

4: Wizards
000-20000-310-02-201312 45
Quick View Minus Background
Select Quick View mode to take a Quick View and subtract the stored background spectra from each
spectrometer channel before OceanView displays it.
►
Procedure
1. Invoke the Quick View Minus Background wizard by clicking the Create new spectroscopy
application icon .
2. Select either an active acquisition or start a new one.
3. Click Spectroscopy, then Quick View Minus Background, then Next.
4. Set Acquisition parameters.
5. Store background spectrum.
Absorbance
Absorbance spectra are a measure of how much light a sample absorbs. For most samples, absorbance
relates linearly to the concentration of the substance. OceanView uses an equation to determine the
concentration of a species in solution (illustrated below). The software uses this equation to evaluate each
pixel on the detector and produce the absorbance spectrum:
Where:
S = Sample intensity at wavelength
D = Background intensity at wavelength
R = Reference intensity at wavelength
The concentration of a species in a solution directly affects the absorbance of the solution. This
relationship, known as Beer’s Law, is expressed as:
Where:
A = Absorbance at wavelength ,
= Extinction coefficient of the absorbing species at wavelength
c = Concentration of the absorbing species and l is the optical path length of the absorption.