Bio-Rad Bio-Plex Software® Upgrades and Conversions - Manual (Page 90)

Bio-Plex Manager Software 6.1 User Guide | Preparing Protocols
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Step 4. Enter Standards Info
This section describes how to enter information about your standards. To
enter information for standard wells defined on the current plate, make sure
the Standards Info tab is selected.
Standards are analytes of known concentration. They are used to generate a
standard curve of values using one of the several regression methods built
into Bio-Plex Manager. This curve is used to calculate the concentrations of
your unknowns.
The standards you select depend on the performance characteristics of your
assay, and the type of curve-fitting model you want to use. Due to variations in
assay preparation, we recommend using a minimum of eight standard
concentrations to generate the standard curve. See the appropriate Bio-Plex
Assay Instruction Manual for recommendations of standard concentrations.
To see what manuals are available, visit
www.bio-rad.com/bio-plex.
The minimum number of non-zero standards required for each type of
regression method available in the software is shown in the following table.
Before entering the concentration values of your standards, you must select
the analytes that you will be analyzing in the reading as described on page 59.
If your current plate contains your standards, you must also identify the
standard wells as described on page 72.
Method Minimum number of standards
Logistic-5PL 6
Logistic-4PL 5
Cubic spine 4
Linear (linear and semi-log) 2
Point to point (linear and semi-log) 2
BioPlex_6.book Page 78 Friday, September 23, 2011 11:55 AM