Bio-Rad Bio-Plex Software® Upgrades and Conversions User Manual
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Bio-Plex Manager Software 6.1 User Guide | Glossary
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Concentration in range
A measure of unknown, standard, and control concentrations that are Conjugated A microsphere set that has been chemically modified so that its surfaces Cytokine An immunological protein that can signal a response in the immune Detection antibody An antibody used in capture sandwich immunoassays that binds a Doublet Two microspheres that have associated. The resulting signal of the Doublet discriminator A channel that measures the amount of light scatter from particles that Dynamic range The calculated number of decades covered by the reporter channel Emission spectrum The wavelength range of light emitted by an excited fluorochrome when Ex/Em A common way of reporting a molecule's excitation and emission Excitation spectrum The wavelength range that excites a molecule's electrons to a higher External standard A standard that has been imported from a different file. Fit probability A statistical measure of goodness of fit that incorporates the residual Fluidics validation The process of verifying the well-to-well carryover of beads in the array Fluorescence The emission of light that occurs when the electrons of a fluorochrome Fluorochrome A fluorescent molecule. Gate An electronic method of discriminating or eliminating aggregated Term Definition BioPlex_6.book Page 236 Friday, September 23, 2011 11:55 AM
reliable, based on the recovery range of valid standards. Values outside
the range are flagged as >OOR or
microsphere
are covered with molecules that bind with the target analytes in an assay
system.
system.
captured analyte to a fluorochrome, allowing for quantitation of the
protein. Detection antibodies may be biotinylated, which allows for
addition of a streptavidin-bound fluorochrome, or the detection antibody
may be directly labeled with a fluorochrome.
associated microspheres is twice that of a single microsphere (singlet).
flow past the red laser. Light scatter is directly proportional to particle
size, and the channel is designed to identify particles that are smaller or
larger than a single microsphere, including microspheres that are
clumped or aggregated.
scale of the array reader. Range is calculated using the Reporter
Validation Kit.
its electrons fall from a higher to a lower energy state. Expressed in
nanometers (nm).
wavelengths (for example, Ex/Em = 488/520 nm).
energy state. Expressed in nanometers (nm).
SSE (sum of the square of the error). A fit probability of 1 indicates a
perfect fit, while a fit probability of 0 indicates no fit.
reader.
drop to a lower energy state.
microspheres in the analysis of an assay. A gate separates singlet beads
from aggregated beads.