Norm ratio, Norm ratio’ norm ratio – Bio-Rad Bio-Plex Software® Upgrades and Conversions User Manual
Page 236
Bio-Plex Manager Software 6.1 User Guide | Appendix
224
Norm Ratio
The calculation is a simple division of each sample’s calculated value by the
control sample’s calculated value.
When internal controls are not chosen:
Norm Ratio
(sample)
=
When internal controls are chosen:
Norm Ratio’’
(sample x)
=
Norm Ratio’
When internal controls are chosen and no control samples are identified:
Norm Ratio’
(well)
=
To figure Norm Ratio’ per sample, use the average normalized value of each
sample.
Norm Ratio’
(sample)
=
Norm Ratio’’
Calculating Norm Ratio” allows the researcher to express all data relative to
the control, so that data across experiments using the same control samples
can be compared.
When control sample and housekeeping genes (internal controls) are chosen:
Norm Ratio’’
(sample x)
=
BioPlex_6.book Page 224 Friday, September 23, 2011 11:55 AM