Bio-Rad Bio-Plex Software® Upgrades and Conversions - Manual (Page 227)

Bio-Plex Manager Software 6.1 User Guide
215
Troubleshooting
Follow these suggestions to troubleshoot your Bio-Plex system.
Message/Problem Cause Solution
“Bio-Plex Manager 6.1
software has detected
a problem with low
bead number” More Likely:
Too few beads in the assay
(2,500 per region recommended). Check bead number calculations in
assay.
Plate not shaken for 30 seconds
before analysis. Remove plate from array reader and
shake for 30 seconds.
Buffer volume in wells is too low
(125 μl is recommended). Resuspend wells in 125 μl. Perform
Remove Bubbles.
Microbubble in cuvette. Perform Remove Bubbles. Perform
Unclog to verify fluidics integrity.
Low/no sheath fluid. Refill sheath fluid, check sheath
connections. Perform Start Up.
Possible clog. Perform Unclog and rerun. If
unsuccessful, repeat. If still
unsuccessful, contact Technical
Support.
Less Likely:
Incorrect needle height. Adjust needle height.
Incompatible plate type used. Replace with flat bottom or filter plate
and adjust needle height.
Vacuum system not calibrated. Calibrate vacuum system (see hardware
manual).
Red laser failure. Contact Technical Support.
Filter plate not flat. Check filter plate flatness (see hardware
manual).
Incompatible suspension buffer
used. Check buffer compatibility (see
hardware manual).
“Bio-Plex Manager has
detected a problem
with selection of
regions” Incorrect bead regions were
selected in the Protocol. Compare bead regions in the assay
with those selected in the Protocol.
Incorrect regions were selected
when preparing the assay. Verify regions chosen during assay
preparation.
Too few beads in the assay in
one or more regions. Verify regions chosen during assay
preparation.
Too few beads in the assay in
one or more regions. Verify that the correct number of beads
was used by referring to the specific
assay preparation procedure.
BioPlex_6.book Page 215 Friday, September 23, 2011 11:55 AM