Bio-Rad Bio-Plex Software® Upgrades and Conversions - Manual (Page 41)

Calibration
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Calibration
Calibration of the array reader is essential for optimal performance and day-
to-day reproducibility of results. Calibration is required:
• Each day after the startup procedure is complete and the optics
have warmed up
• If the array reader temperature changes by more than 2ºC during
the course of the day. (If the temperature changes by more than
2ºC, a message box prompts you to recalibrate)
• Before data acquisition, if you switch between Bio-Plex Manager
and Luminex software installed on the same computer (see page 6)
NOTE: Before calibrating, make sure that optics warm up is complete.
The Bio-Plex Calibration Kit contains calibration microspheres (CAL1 and
CAL2 beads) with stable fluorescent intensities in the RP1, CL1, and CL2
wavelength ranges. The calibration process uses these microspheres to
adjust voltage settings for optimal and consistent microsphere classification
and reporter readings over time and across different instruments. Current
calibrated settings are automatically applied to any new session.
Calibration using Bio-Plex Manager requires the Bio-Plex MCV Plate IV, CAL1
beads and CAL2 beads from the Bio-Plex Calibration Kit, and distilled or
deionized water. The CAL1 beads calibrate the array reader's doublet
discriminator and classification channels, while the CAL2 beads calibrate the
array reader's reporter channel for reporter fluorescence detection.
BioPlex_6.book Page 29 Friday, September 23, 2011 11:55 AM