Bio-Rad Bio-Plex Software® Upgrades and Conversions - Manual (Page 247)

Bio-Plex Manager Software 6.1 User Guide
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Glossary
Term Definition
Accuracy The closeness of a measured value to the actual value or the difference
between expected and measured values. Using the Reporter Validation
Kit, accuracy is defined as the percent difference between the measured
regression curve and the expected or actual data points.
Aggregated
microspheres Microspheres that have associated. Two microspheres that have
associated are called a doublet.
Analyte The substance being measured, identified, quantitated, or otherwise
analyzed in an experiment. In the Bio-Plex system, analytes are
molecules that are being quantitated.
Antibody Immunological protein capable of binding a unique antigen via a specific
binding site or epitope. Used in immunological assays to quantitate the
amount of a specific analyte present.
Assay The bead and reagent mixture that, when combined with sample, is
loaded into microplate wells and read by the array reader.
Background (noise) Fluorescent intensity measured by the reporter channel that is due to
non-specific binding of a fluorochrome and the electronic noise of the
array reader. If you include blank wells in your microplates, you can
calculate the average background intensity and subtract it from the
intensity readings of your standards and unknowns.
Bead A microsphere.
Calibration A process that uses microspheres (calibrators) with a stable fluorescent
intensity to adjust the gain settings in the array reader's detectors for
optimal and consistent microsphere classifications and reporter
readings, over time and across different array readers.
Calibrators Microspheres with stable fluorescent intensities used to standardize the
signal output of the array reader.
Capture antibody An antibody used in sandwich immunoassays that binds or "captures" a
specific analyte for quantitation.
Carboxylated
microspheres Microspheres with chemically modified surfaces covered with carboxyl
functional groups (COOH) that allow covalent biomolecular attachment.
Classification channels Fluorescent detection channels of the array reader that detect the
fluorescent intensities of two dyes embedded in each microsphere. If the
signals from the two dyes place the bead within a specified classification
region, the bead is identified as a unique analyte in a sample.
Classification regions Defined regions in a dot plot bead map that are used to identify beads in
an assay based on the fluorescent signals from their embedded dyes.
Classify validation A validation procedure for measuring the ability of the array reader to
efficiently classify assay beads into specified regions.
Clumping The aggregation of two or more microspheres.
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