Bio-Rad Bio-Plex Software® Upgrades and Conversions - Manual (Page 125)

Histogram and Bead Map
113
D OUBLET D ISCRIMINATOR G ATE R ANGE
The Doublet Discriminator (DD) range specified under the
Run Protocol Advanced Settings (see page 107) is shown
on the histogram as two red lines perpendicular to the x-
axis. In the Protocol window, you can change the DD gate
range by positioning your cursor over the red lines and
dragging them to the right or left. This also changes the
range as listed in the Advanced Settings dialog.
NOTE: The DD gate range cannot be changed while the
Protocol is running.
Figure 101. Changing the DD gate range
You can show or hide the gate lines by right-clicking on the
histogram and selecting Show or Hide from the Gate context menu.
To reset the gates to their default values, click the Restore Default Gates
button above the histogram.
S ELECTING THE C HANNEL T YPE
You can change the histogram to display different channel types. Click the
histogram with the right mouse button and select X-Axis from the context
menu.
Figure 102. Histogram right-click context menu
From the X-Axis submenu, select from the following channel types:
• The Doublet Discriminator channel measures the forward light
scatter from particles as they pass through the red laser. Light
scatter correlates to particle size; this channel allows you to
discriminate single beads (“singlets”) from aggregated beads in the
histogram. This is the default selection
BioPlex_6.book Page 113 Friday, September 23, 2011 11:55 AM