Bio-Rad Bio-Plex Software® Upgrades and Conversions - Manual (Page 215)

Determining Outliers
203
C OMMENTS
Comments are included to point out particular issues that may be of interest.
• Low Bead Counts Detected (Low Bead)—This message will be
presented if fewer than 20 beads are detected. This suggests one
of the following problems:
- Incorrect gates are set
- Incorrect region is set
- Insufficient agitation of plate before reading
- Perforated well (if using a filter plate)
- Some other contributor to bead loss
• Standard Concentrations are not set (Stand Conc)—Standards
have either no value of 0 value entered. Correct this by applying the
appropriate concentration to your standards, or renaming your 0
standard as a blank (it is inappropriate to have a standard of 0
value).
• One or more standard has a CV higher than 20% (High % CV) - If
one or more standard has a CV higher than 20%, you may want to
check your data to identify systemic loading issues. Also make
sure you agree with the algorithm decisions to mark certain
standards as outliers.
• More than one standard (all replicates) has been marked as an
outlier (>1 Std Removed) - This message will be displayed if all
replicates of more than one standard point has been completely
removed (that is, marked as an outlier). You may want to review the
data for such standard curves.
• The upper range of your curve is limited because 2 or more high
concentration standards have poor recovery.
By default, the report displays at the end of an optimization. You may disable
the automatic display by deselecting the checkbox next to Show report after
optimization. This report can also be viewed at any time, by choosing the
button below the optimizer controls.
BioPlex_6.book Page 203 Friday, September 23, 2011 11:55 AM