7 running protocols, Chapter 7. running protocols, Running protocols – Bio-Rad Bio-Plex Software® Upgrades and Conversions User Manual
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Bio-Plex Manager Software 6.1 User Guide
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7
Running Protocols
After you have prepared the Protocol as described in the previous chapter,
you are ready to run it.
Click the Run Protocol button
in the Protocol window to select the final
settings and run the Protocol.
WARNINGS:
•
When performing the following operations, the sheath fluid
container and the waste fluid container should be closely
monitored
•
The sheath fluid bottle must be placed at the same level as the
array reader, unless you are using the HTF (High-Throughput
Fluidics, (see the next Warning). The fluid level should be below the
air inlet connection and above the sheath outlet connection. For
more information, refer to the Bio-Plex 200 System Hardware
Instruction Manual. Always check the sheath fluid level before
starting a run or procedure
•
If you are using the HTF, it should sit on the counter next to the
array reader, while the sheath fluid cube should be placed ~3–4
feet below the reader (for example, on the floor)
•
The waste fluid container receives waste from the system. Do not
allow the waste container to overflow. Empty the waste bottle each
time the sheath fluid bottle is filled. Do not place it on the
instrument. All waste containers should have vented caps.
BioPlex_6.book Page 99 Friday, September 23, 2011 11:55 AM