Bio-Rad Bio-Plex Software® Upgrades and Conversions - Manual (Page 126)

Bio-Plex Manager Software 6.1 User Guide | Running Protocols
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• The Reporter 1 channel measures fluorescence from the reporter
molecules bound to the analytes on each bead as they pass
through the green laser. The amount of fluorescence per bead type
is directly proportional to the amount of analyte present in the
assay. This can be useful for viewing the distribution of the
fluorescence signal of a particular analyte
• The Classification 1 channel measures fluorescence from the first
classification dye embedded in each bead as it passes through the
red laser
• The Classification 2 channel measures fluorescence from the
second classification dye embedded in each bead as it passes
through the red laser
NOTE: Changing the channel type changes the channel numbers and the
histogram display.
S CALING THE H ISTOGRAM D ATA
If the histogram is too big or too small to fit comfortably within the display
range, click the Auto Scale button on the histogram toolbar. The height of
the y axis changes to better fit the data. You can also select this command
from the right-click context menu.
Select Set Scale from the right-click histogram context menu to manually set
the range of the y-axis. In the pop-up box, enter the maximum number of
events to display on the y-axis between 20 and 100,000.
The default view of the data is log scale. From the toolbar, click the Log/Linear
button to change the display from logarithmic to linear. Click again to
return to log scale.
M AGNIFYING AND R ESIZING THE H ISTOGRAM
Click the Zoom button on the histogram toolbar to magnify the display.
With Zoom selected, the cursor changes to a hand symbol when you
position it over the histogram. Drag the hand cursor to view different
magnified regions of the histogram.
BioPlex_6.book Page 114 Friday, September 23, 2011 11:55 AM