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Bio-Rad Bio-Plex Software® Upgrades and Conversions User Manual

Page 229

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217

“Check Link” in status
bar of software

Array reader or microplate
platform is not turned on.

Turn on array reader and microplate
platform.

Software is not communicating
with array reader.

Close and restart Bio-Plex Manager.

Cables between computer and
array reader or microplate platform
are loose/not connected.

Check cables for proper connections.
See System cable connections on
page 16.

“Pressurizing” in
software status bar

Leak in sheath bottle or cap.

Tighten sheath cap.

Platform heater not at
target temperature

Insufficient warm-up time
allowed.

Allow at least 15 minutes for platform
heater to reach target temperature. If
heater does not reach target in 30
minutes, contact Technical Support.

Platform heater not at
ambient temperature

Platform heater not functioning
properly.

Contact Technical Support.

Insufficient cool-down time
allowed.

Open platform door to facilitate cooling.
An ice pack can also be placed on the
platform plate holder to facilitate
cooling.

No Assay Signal
Detected

More likely:

Sheath reservoir cap not on
securely.

Tighten sheath cap. Message should
disappear within two minutes.

Sheath bottle lines are not
connected properly.

Make sure that all hoses are connected
to the appropriate ports, and that they
have clicked into place.

Less likely:

Sheath fluid level above the AIR
port on the sheath container.

Adjust sheath fluid level so that sheath
fluid is below the AIR port of sheath
bottle.

Sheath bottle has a leak.

Try new sheath bottle. Call Technical
Support for further assistance.

High-throughput fluidics (HTF)
system not turned on.

Turn on HTF and check to ensure that
message disappears.

Air Compressor not working.

List for air pump to turn on when Warm
Up is selected. Contact Technical
Support for further assistance.

Bio-Plex Manager has
detected a change in
array reader tempera-
ture. Please calibrate
before running an
assay to ensure
accurate results.

Room temperature has changed. Calibrate array reader.

Message/Problem

Cause

Solution

BioPlex_6.book Page 217 Friday, September 23, 2011 11:55 AM