Bio-Rad Bio-Plex Software® Upgrades and Conversions - Manual (Page 251)

239
Reporter A fluorescent molecule that is incorporated into an assay in such a way
that the fluorescence intensity is directly proportional to the amount of
analyte in an assay.
Reporter channel The channel of the array reader that detects the fluorescent signal of the
reporter molecule (phycoerythrin).
Reporter system Any combination of molecules in solution that serves to quantitate the
analytes in a particular assay.
Reporter validation A standard method for verifying the performance of the reporter channel
of the array reader. The five primary parameters that verify accurate
performance of the reporter channel include linearity, dynamic range,
sensitivity, accuracy, and slope of the response.
Residual variance A statistical measure of a good fit. This is the weighted sum of the
squared errors between the observed response and the response
predicted by the fitted curve (residual SSE), divided by the number of
degrees of freedom. A small residual variance indicates a good curve fit.
Results file (*.rbx or
*.srbx) A file containing the results of a reading, including the raw data, the
settings information from the Protocol, and tools for analyzing and
exporting the data (tables, a standard curve, export functions, etc.). Each
reading generates a new Results file. In Bio-Plex Manager Security
Edition, Results files may be Secure files, in which case they have the file
extension *.srbx.
Sample A solution of analyte standards or unknowns that, when added to an
assay, will bind to the microspheres in that assay and be identified and
quantitated by the array reader.
Sampling error A measure of the degree to which a sample differs from the entire
population.
Sandwich immunoassay Immunoassay for the quantitation of a specific analyte through the use of
monoclonal antibodies (Ab). A sandwich is formed when an analyte of
interest binds to a capture Ab, and then a second antibody (detection
Ab) binds to a different epitope of the protein.
Secure file A Protocol or Results file that has been electronically "signed" by a user.
Once signed, this file is a controlled document that cannot be
overwritten by Bio-Plex Manager and that preserves a built-in audit trail
of all saved changes. If you make changes to a Secure file and save it as
a new file, the new file will not be signed; however, the audit trail will be
preserved in the new file and the file will remain a Secure file.
Sensitivity The lowest detectable signal above instrument noise. Noise may be
attributed to the lasers, the detectors and the amplification electronics of
the array reader.
Signal The detectable measurement unit of a reporter molecule.
Signal-to-noise ratio The ratio of a specific assay signal to the underlying noise in the assay.
Signal to noise is typically used to measure the sensitivity of an assay.
Singlet(s) A single microsphere or a population of single microspheres. Compare
to doublets which are two microspheres associated together.
Term Definition
BioPlex_6.book Page 239 Friday, September 23, 2011 11:55 AM