Metrohm 789 Robotic Sample Processor XL - Manual (Page 138)

6.2 Standard methods
130 Metrohm Sample Processor, Annex
STIR T1: cont.
STIR T2: cont.
STIR MSB1: cont.
STIR MSB2: cont.
STIR MSB3: cont.
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- Do not alter stirrer status
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"
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6.2.2 PIP ext
Standard method for pipetting samples into an external titration cell.
Very suitable for standard titrations.
Required instruments and cables:
• Metrohm titrator (Titrino or Titrando with 6.2148.010 Remote Box)
• 786 Swing Head with robotic arm for sample transfer (6.1462.030 or
6.1462.040)
• 803 Magnetic Stirrer or 804 Ti Stand with 802 Rod Stirrer connected
to MSB1
• 800 Dosino with 807 Dosing unit (recommended: 10 mL cylinder,
6.3032.210) and 6.1562.100 Pipetting tubing
• 6.2141.020 Remote cable
Special features:
Samples are pipetted directly from the sample beaker into the external
titration cell. The external Position 1 with the working height for moving
to the titration cell must be set in the tower configuration. No special
beaker is required.
789 Sample Proc. XL 11122 5.789.0011
parameters
method PIP ext
number of samples: rack
>start sequence
1 CTL:Rm: INIT
2 MOVE 1 : ext.1
3 LIFT: 1 : rinse mm
4 PUMP 1.2 : 30 s
5 DOS: 1.1 : prepar. mL
6 PUMP 1.* : 30 s
7 LIFT: 1 : shift mm
>sample sequence
1 MOVE 1 : ext.1
2 LIFT: 1 : work mm
3 PUMP 1.2 : ON s
4 DOS: 1.1 : endVol mL
5 DOS: 1.1 : compen. mL
6 PUMP 1.2 : OFF s
7 LIFT: 1 : shift mm
8 DOS: 1.1 : -0.5 mL
9 MOVE 1 : sample
10 LIFT: 1 : work mm
11 DOS: 1.1 : -5.0 mL
12 WAIT: pause 3 s
13 LIFT: 1 : shift mm
14 DOS: 1.1 : -0.2 mL
15 DOS: 1.2 : port mL
16 MOVE 1 : ext.1
17 STIR: MSB1 : ON s
18 PUMP 1.1 : 10 s
19 LIFT: 1 : work mm
- Report header with device ID and program version
- Method name
- No. of samples (here whole sample rack)
- Initialize Remote interface
- Move robotic arm to external titration cell
- Run lift to rinsing height
- Rinse for 30 seconds
- Prepare Dosing unit
- Rinse titration cell for 30 seconds and aspirate
- Run lift to shifting height
- Move robotic arm to external titration cell
- Run lift to work height
- Rinse cell, pump on
- Eject all dosing cylinder contents
- Eliminate slack
- Pump off
- Run lift to shifting height
- Aspirate air-gap bubble into pipetting tubing
- Rotate rack to sample beaker
- Run lift to working height
- Aspirate sample into pipetting tubing
- Wait for pressure equilibration in pipetting tubing
- Run lift to shifting height
- Aspirate air into pipetting tip
- Rotate Dosino stopcock, close Port 1
- Move robotic arm to titration cell
- Switch on stirrer
- Add solvent with pump
- Run lift to working height