Step 4. specify output – Bio-Rad Image Lab™ Software User Manual
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| Acquiring Images
82 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab Software
Gradient gels — the linear (semilog) regression method works well for
these gels because the mobility of the bands is linear to the log of their
molecular weight. As an alternative, the point-to-point (semilog) method
can be used if the R
2
value is not sufficient.
Fixed percentage gels — these gels have a nonlinear relationship
between the gels’ mobility and their molecular weight. For these gels,
choose the logistic or cubic spline regression method.
For more information about regression methods, see
.
Step 4. Specify Output
To specify the output of the protocol
1.
Select Specify Output in the left pane of the Protocol Setup window to display
output options.
2.
In the right pane, you can choose to
Automatically print the image