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Bio-Rad Image Lab™ Software User Manual

Page 110

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7

| Viewing Images

110 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab Software

The zoom tools work the same way they work elsewhere in the software. For more

information, see

Zoom Tools on page

99

.

In addition to the graph of the lane intensities, the Lane Profile tool also shows an

image of the selected lane below the graph. The transform and color map are

applied to the gray-scale image.

As you move your cursor over the profile, the current relative front (Rf) value and the

average intensity (Int) value at the Rf value display in the lower-right corner of the

Lane Profile window.

Scale to Fit Graph

You can choose the highest point of the display to define the range of the graph.

This provides the best view of the lane profile.

You can clear the Scale to Fit Graph checkbox to display the entire range of

possible intensity values in the graph. Doing so allows valid comparisons between

different lanes.

Include Background

When the Include Background checkbox is selected, the Lane Profile window

shows the subtracted background under the blue line. The area used for band

quantification appears in green under the red line.

When you clear the Include Background checkbox, the area of the lane profile that

represents the background of the image does not display.

Identify Bands by

You can change how the bands are labeled by choosing from the options in the

Identify Bands by dropdown list. By default, the bands are labeled by band number.

You can display one of the following attributes:

Band Number (Band No.)

Band Label

Molecular Weight (Mol. Wt.)

This manual is related to the following products: