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Step 3. analyze molecular weight – Bio-Rad Image Lab™ Software User Manual

Page 79

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Creating a Protocol

User Guide

| 79

Custom — select a numeric value from 1 to 100 to choose the best

detection sensitivity for your sample.

Step 3. Analyze Molecular Weight

Determining molecular weight depends on selecting the proper protein standards.

Many protein standards are available from Bio-Rad and many different DNA

standards are also available. See

Appendix D, Accessories

for all Bio-Rad standards

and their catalog numbers.

To specify how molecular weight is analyzed

1.

Select the Analyze Molecular Weight checkbox in the left pane of the Protocol
Setup window.

The software calculates the molecular weight for each band based on the

specified standard.

2.

To estimate the size of the molecules in the bands of your gel, enter the
standard you are using.

Alternatively, you can choose from available standards:

This manual is related to the following products: