Leica Biosystems CV5030 - Manual (Page 92)

92 Instructions for Use V 3.0 RevE – 04/2013
8. Malfunctions and Troubleshooting
Problem Possible cause Remedy
Air bubbles (between
specimen and cover slip).
The dispenser needle diameter has
not been selected appropriately for
the coverslip mountant used.
The size of the dispenser needle has to be selected
according to the list of recommendations ( refer to
Chapter 5.12 ) of the various coverslip mountants or be
determined as described in the configuration recom-
mendations.
Air bubbles (between
specimen and cover slip).
The air bubbles are produced by
leaks in the hose system of the
dispenser group.
If there is a leak in the hose system that cannot be rem-
edied, notify the responsible Leica Service.
Air bubbles (between
specimen and cover slip).
The coverslip mountant is incom-
patible with the solvent used.
Make sure that a solvent compatible with the coverslip
mountant is used in the loading bath of the CV5030 as
well as in the last steps of the preceding dyeing process.
If commercially available coverslip mountant is diluted
with a solvent that is incompatible, this can also cause
small air bubbles.
Incompatibility can frequently be identified by striation.
Air bubbles (between
specimen and cover slip).
The suction cups of the Pick &
Place module have become stuck
or deformed.
Non-functional suction cups impede coverslip placement.
Check the suction cups for glued-on material and defor-
mation and replace them if necessary.
If material has become glued-on because of incor-
rectly configured parameters (such as too much coverslip
mountant), these have to be checked and readjusted.
No coverslip mountant is
applied to the specimen
slide.
1. The dispenser needle is com-
pletely clogged.
2. The plug ( Chapter 4.6, Fig. 8 , 38) is
detached.
1. The dispenser needle is partially or completely clogged
by dried coverslip mountant. Replace the clogged
needle with a new one of the same size.
2. Insert the plug.
Coverslip mountant is
applied over the entire
length of the specimen
slide, including the label
field.
1. Height of the dispenser needle is
set incorrectly.
2. The dispenser needle diameter
has not been selected appropri-
ately for the coverslip mountant
used.
3. The coverslipping parameters are
incorrect.
1. Set the height of the dispenser needle correctly.
2. and 3. The dispenser needle size and coverslipping
parameters have to be selected in accordance with
the list of recommendations of various coverslip
mountants ( refer to Chapter 5.12 ) or be determined as
in Chapter 5.13 .
8.2 Troubleshooting (continued)