Leica Biosystems CV5030 - Manual (Page 67)

67 Leica CV5030 – Robotic Coverslipper
5. Operation
5.13.1 Procedure (continued)
• In contrast to "wet" coverslipping, higher ap-
plication quantities of mountant are necessary
for "dry" coverslipping, i.e. the parameters VOL
and/or TYP must be increased as needed.
• If this is no longer possible, a dispenser needle
with larger diameter can also be used.
4 Preparing the coverslipping machine
• Be mindful of air-tightness when insert-
ing the coverslip mountant bottle ( refer to
Chapter 4.11 ).
• Insert a suitable dispenser needle ( refer to
Chapter 4.6 ).
• Check the dispenser needle height and adjust
as needed ( refer to Chapter 4.7 ).
• Carry out the priming cycle ( refer to
Chapter 5.3 ).
• Fill the dispenser needle cleaner, being mindful
of the correct seating ( refer to Chapter 4.8 ).
• Fill the loading bath with a suitable solvent
( refer to Chapter 4.11 ).
• Fill the cover slip magazine ( refer to
Chapter 4.10 ).
5. Important information regarding test run
procedure for determining the optimum cov-
erslipping parameters
6. Evaluation of coverslipping quality
The respective coverslipping quality of the test
runs should be checked at various points in time
for adhesive and air bubbles:
•
Directly after coverslipping operation.
• 1 - 3 hours after coverslipping.
• 24 - 48 hours after coverslipping.
Should air pockets appear between the speci-
men slide and cover slip after these checks, the
settings must be corrected.
If air pockets still occur despite suitable chang-
es, Chapter 8.2 provides additional directions.
• The initial coverslipping trials
should be carried out with empty
specimen slides, i.e. without a spec-
imen.
• If coverslipping quality is good, sub-
sequently carry out a test series us-
ing an applied specimen.
• Since factors such as section thick-
ness and specimen composition and
type can affect the coverslipping
quality, it may be necessary to make
minor corrections to the parameter
settings.
Ex. VOL: Increase or reduction of ad-
justment by ± 1
and/or TYP: Increase or reduction of
adjustment by ± 1