Leica Biosystems CV5030 - Manual (Page 71)

71 Leica CV5030 – Robotic Coverslipper
5. Operation
Notes on fine-tuning the parameters STP, CSP, LEN:
5.13.1 Procedure (continued):
• In most cases, the parameter STP must be set
in the positive range at approx. +20 to +40 for
extremely low-viscosity coverslip mountants
(information is based on experimental values
and may vary).
For lower values (negative value up to 0/+10),
there is the danger that the coverslip moun-
tant will flow out over the specimen slide or
coverslip edge.
On the other hand, if the starting point of the
coverslip mountant stroke is still positioned
too far towards the specimen slide center, air
pockets could form in the coverslip edge area
after coverslipping and drying of the coverslip
mountant.
• The coverslip placement CSP should be posi-
tioned before starting the coverslip mountant
stroke (in relation to the specimen slide edge,
which faces the label field). The settings are
to be customized based on the properties of
the coverslip mountant used, whether the
coverslipping is "wet" or "dry," and the speci-
men slide used. If using specimen slides with
"clipped corners" (i.e. all four corners are
ground off or rounded), take care that the
corners do not overlap the cover slip.
If the coverslip placement is not optimal, air
bubbles or glued-on material may result at the
suction cups.
• The length correction LEN shortens the cov-
erslip mountant stroke from the area of the
specimen slide label field towards the center
of the specimen slide.
If, even though you have the application quan-
tity at a nearly optimal setting (STK, VOL, TYP),
you identify excess coverslip mountant in the
area of the coverslip edge, you can correct
this excess by changing the setting for LEN.
The application quantity of the coverslipping
mountant remains constant during this pro-
cess. Only the length of the strip of coverslip
mountant is shortened. This also makes it
possible to correct small bubbles in the area
of the coverslip edge near the label field.