Leica Biosystems CV5030 - Manual (Page 88)

88 Instructions for Use V 3.0 RevE – 04/2013
7. Cleaning and Maintenance
7.8 Procedure for changing the cover slip mountant
7.8.1 Changing from one xylene-based
coverslip mountant to another
• Remove the mountant bottle.
• Insert a second glass bottle with 150 ml of
xylene and rinse/prime it (see Chap. 7.3 B).
• Insert a mountant bottle with the new moun-
tant.
• Run a prime cycle. Hold down the PRIME
button until the hose is completely free of air
bubbles and filled with coverslip mountant.
7.8.2 Changing from a xylene substitute
coverslip mountant to a xylene-based
mountant
• Remove the mountant bottle.
• Insert a second glass bottle with 150 ml of
xylene substitute and rinse/prime it (see
Chap. 7.3 B).
• Empty the second glass bottle and fill it with 150 ml
of 100 % ethanol before inserting it and rinsing.
• Empty the second glass bottle and fill it with
150 ml of xylene before inserting it and rinsing/
priming.
• Insert a mountant bottle with the new mountant.
• Run a prime cycle. Hold down the PRIME
button until the hose is completely free of air
bubbles and filled with coverslip mountant.
7.8.3 Changing from a xylene-based
coverslip mountant to a xylene
substitute
The sequence of individual substanc-
es has to be followed to avoid milky/
cloudy clumping in the hose and the
dispenser group.
All parts that were filled with xylene
must be converted to the substitute
(= loading bath, glass vial, dispenser
needle cleaner)!
• Remove the mountant bottle.
• Insert a second glass bottle with 150 ml of
xylene and rinse/prime it (see Chap. 7.3 B).
• Empty the second glass bottle and fill it with
150 ml of 100 % ethanol before inserting it and
rinsing.
• Empty the second glass bottle and fill it with
150 ml of xylene substitute before inserting it
and rinsing/priming.
• Insert a mountant bottle with the new, substi-
tute-compatible mountant.
• Run a prime cycle. Hold down the PRIME
button until the hose is completely free of air
bubbles and filled with coverslip mountant.
Before switching the mountant, switch
off the instrument and disconnect it from
the power supply! Remove the loading
bath and specimen slide holders from
the instrument before switching it off.
Due to the high pressure when rinsing
with solvent, it is necessary to wear
gloves, safety goggles and appropri-
ate protective clothing when changing
the cover slip mountant! Select an ad-
equately sized container to catch the
rinsing residue.