Leica Biosystems CV5030 - Manual (Page 66)

66 Instructions for Use V 3.0 RevE – 04/2013
5.13 Determining the optimum parameter setting (menu A+B)
5. Operation
The following procedure provides assistance for:
• Adjusting the previously listed parameter set-
ting to onsite lab conditions.
• Determining the optimum parameter settings
for an unlisted coverslip mountant ( refer to
Chapter 5.12 ).
• Changing from coverslipping with solvent
("wet") to coverslipping without solvent ("dry")
in the loading bath, or, reversed ( refer to
Chapter 5.13.1 - 3. Differences between "wet"
and "dry" coverslipping ).
The following sections give a detailed descrip-
tion of the steps to carry out, from the instrument
preparation through to specimen evaluation. Dur-
ing the test series for determining the optimum
parameter sets, the coverslipping operation in
the instrument should also be observed.
5.13.1 Procedure
1. Filling the coverslip mountant
• It is imperative that there be no air bubbles in
the coverslip mountant during parameter set-
ting (use clean bottles; refer to Chapter 4.11 )
2. Selecting the suitable size or number of
dispenser needles (nozzle)
=> The following are suitable for high-viscosity
coverslip mountants:
• Dispenser needle No. 16
(largest diameter),
• Dispenser needle No. 18
=> The following are suitable for low-viscosity
coverslip mountants:
• Dispenser needle No. 21
(smallest diameter),
• Dispenser needle No. 20
=> The following are suitable for mid-viscosity
coverslip mountants:
• Dispenser needle No. 18
• Dispenser needle No. 20
Dispenser needle 21 (smallest diameter) =>
dispenser needle 20 => dispenser needle 18 =>
dispenser needle 16 (largest diameter).
3. Differences between "wet" and "dry" cover-
slipping
"Wet" coverslipping with filled loading bath:
• The loading bath must be sufficiently filled
with a solvent compatible with the coverslip
mountant, i.e. the solvent must extend to the
label field of the specimen slide used in the
loading bath.
Or:
"Dry" coverslipping with unfilled loading bath:
Note: