Leica Biosystems CV5030 - Manual (Page 63)

63 Leica CV5030 – Robotic Coverslipper
5.12 Recommendation for parameter setting (beginning with firmware 3.01.04)
5. Operation
The following settings serve as recommendations and have to be
adapted to the conditions in the respective lab.
The parameter settings have been determined as follows under
standardized condition in a Leica Biosystems Nussloch GmbH lab:
• Use of tissue sections with a thickness of 3-5 µm.
• Room temperature: 20-25 °C.
• Use of cover slip with a size of 24 x 50 mm and thickness #1.
• Filling level for the loading bath reaches up to the label field of
the loaded specimen slide.
• Filling the coverslip mountant bottle with 150-170 ml of coverslip
mountant.
The STP (starting point for the coverslip mountant stroke) and CSP
(cover slip placement position) parameters are mentioned only in
exceptional cases or not at all.
They have to be adapted on a case-by-case basis.
Recommendations for settings for earlier firmware ver-
sions (before firmware version 3.01.04) can be requested
from your local Leica Biosystems representative.
We recommend using only consumables validated by
Leica!