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Procedure–salt water – LaMotte SMART Spectro Spectrophotometer User Manual

Page 69

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To determine the percentage of Ammonia-Nitrogen that is unionized and ionized,

consult the Appendix.

PROCEDURE–SALT WATER

Use universal sample holder.

1. Press and hold ON button until spectrophotometer turns on.
2. Scroll to and select PROGRAMMED TESTS from menu.
3. Scroll to and select ALL TESTS (or another sequence containing

4 Ammonia-N LS) from TESTING MENU.

4. Scroll to and select 4 Ammonia-N LS from menu.
5. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with

sample.

6. Insert tube into chamber, close lid and select SCAN BLANK. (See Note.)
7. Remove tube from Spectro. Use the 1.0 mL plastic pipet (0354) to add 2.0 mL

of *Salicylate Ammonia #1 (3978). Cap and mix.

8. Use the 0.15 g spoon (0727) to add two measures of *Salicylate #2 Reagent

(7457). Cap and mix until dissolved. Wait 1 minute.

9. At end of 1 minute waiting period use 0.1 g spoon (0699) to add two measures

of *Salicylate #3 Reagent Powder (7458). Cap and shake vigorously for at

least 30 seconds and all solid has dissolved. Wait 20 minutes for maximum

color development.

10. At the end of 20 minute waiting period, mix, insert tube into chamber, close lid

and select SCAN SAMPLE. Record result.

11. Press OFF button to turn spectrophotometer off or press EXIT button to exit to

a previous menu or make another menu selection.

AMMONIA-NITROGEN, Low Range

SMART Spectro Test Procedures 2.11

Test P

rocedures