LaMotte SMART Spectro Spectrophotometer - Manual (Page 173)

PROCEDURE
Use universal sample holder.
1. Press and hold ON button until spectrophotometer turns on.
2. Scroll to and select PROGRAMMED TESTS.
3. Scroll to and select ALL TESTS (or another sequence containing
53 Iron Phen) from TESTING MENU.
4. Scroll to and select 53 Iron Phen from menu.
5. Rinse a clean tube (0290)with sample water. Fill to the 10 mL mark with
sample.
6. Insert tube into chamber, close lid and select SCAN BLANK.
7. Remove the tube from Spectro. Remove the cap and add 6 drops of *Acid
Phenanthroline Indicator (2776). Cap and invert the tube 4 times to mix
reagents. Wait fi ve minutes for maximum color development.
8. After fi ve minutes, mix, insert tube into chamber, close lid and select SCAN
SAMPLE. Record result as ppm Ferrous Iron.
9. Remove tube from Spectro. Use the 0.1g spoon (0699) to add one measure
of *Iron Reducting Reagent (2777). Cap and invert 15-20 times to mix, wait 5
minutes for maximum color delelopment.
10. After 5 minutes, mix insert tube into Spectro. Close lid and select SCAN
SAMPLE. Record result as ppm Total Iron.
11. Press OFF button to turn spectrophotometer off or press EXIT button to exit to
a previous menu or make another menu selection.
12. Total Iron (ppm) - Ferrous Iron (ppm) = Ferric Iron (ppm)
NOTE: For best possible results, a reagent blank should be determined to account
for any contribution to the test result by the reagent system. To determine the
reagent blank, follow the above test procedure to scan a distilled or deionized
water blank. Then follow the above procedure to perform the test on a distilled or
deionized water sample. This test result is the reagent blank. Subtract the reagent
blank from all subsequent test results of unknown samples. It is necessary to
determine the reagent blank only when a new lot number of reagents is obtained.
IRON, I,I0-Phenanthroline SMART Spectro Test Procedures 2.11
Test P
rocedures