LaMotte SMART Spectro Spectrophotometer - Manual (Page 212)

NOTE: HR Reagent must be made fresh each week. If reagent is refrigerated, it
may be kept up to 3 weeks.
PROCEDURE–HIGH RANGE
Use universal sample holder.
A. PREPARATION OF HR REAGENT
NOTE: The quantity of Indigo Blue Stock solution (3989) supplied will prepare one
batch of HR Reagent for the High Range Ozone procedure or fi ve batches of HR
Reagent for the Low Range Ozone procedure.
1. Use the 50 mL graduated cylinder to carefully add 35 mL of *Ozone Buffer
(3991) to amber glass bottle marked HR Reagent (0680).
2. Use the 50 mL graduated cylinder to carefully add 15 mL of Indigo Blue Stock
Solution (3989) to the amber glass bottle. Cap and mix.
B. DETERMINATION OF OZONE
3. Use the 1.0 mL transfer pipet (2-2170) and pump (30527) to add 1.0 mL of HR
Reagent to each of 2 clean tubes (0290).
4. If chlorine is present add 3 drops Chlorine Inhibitor (3990) to each tube. Cap
tubes.
5. Take one of the prepared tubes (0290) and sampling apparatus (0681) to
sampling site.
6. Lower end of tubing of sampling apparatus to desired depth. Slowly withdraw
and depress plunger several times to purge syringe and tubing. Slowly
withdraw plunger to fi ll purged syringe.
7. Remove plastic tubing from syringe. Remove cap from the prepared tube.
Place tip of syringe against inside of the prepared tube. Slowly depress
plunger and fi ll to the 10 mL line and cap. This is the Sample Tube.
NOTE: DO NOT SHAKE OR INVERT THE SAMPLE.
8. Fill the second prepared tube (0290) to the 10 mL line with ozone free water.
This is the Reagent Blank.
9. Press and hold ON button until spectrophotometer turns on.
10. Scroll to and select PROGRAMMED TESTS.
11. Scroll to and select ALL TESTS (or another sequence containing
72 OZONE-HR) from TESTING MENU.
12. Scroll to and select 72 OZONE-HR from menu.
13. Insert the Reagent Blank tube into chamber, close lid and select SCAN
BLANK.
SMART Spectro Test Procedures 2.11 OZONE
Test P
rocedures