LaMotte SMART Spectro Spectrophotometer - Manual (Page 251)

PROCEDURE
Use universal sample holder.
1. Press and hold ON button until spectrophotometer turns on.
2. Scroll to and select PROGRAMMED TESTS.
3. Scroll to and select ALL TESTS (or another sequence containing
94 Surfactants) from TESTING MENU.
4. Scroll to and select 94 Surfactants from menu.
5. Rinse a tube (0290) with sample water. Fill to 10 mL line with sample.
6. Insert the tube into chamber, close lid and select SCAN BLANK.
7. Remove the tube from Spectro.
8. Use the 0.5 g spoon (0698) to add 0.5 g pH Adjustment Powder (4509). Cap
and mix until powder dissolves.
9. Use the 0.1 g spoon (0699) to add two measures of Sodium Chloride Reagent
(4877). Cap and mix until powder disintegrates.
10. Use the 1.0 pipet (0354) to add 2.0 mL of *DS Indicator (4508).
11. Cap and shake for 1 minute.
NOTE: Bubbles on the sides of the tube will interfere with the results. Swirl the
tube to remove bubbles if they are present.
12. Wait 5 minutes. DO NOT MIX.
13. Insert tube into chamber, close lid and select SCAN SAMPLE. Record result
in ppm LAS.
14. Press OFF button to turn the spectrophotometer off or press EXIT button to
exit to a previous menu or make another menu selection.
NOTE: For best possible results, a reagent blank should be determined to account
for any contribution to the test result by the reagent system. To determine the
reagent blank, follow the above test procedure to scan a distilled or deionized
water blank. Then follow the above procedure to perform the test on a distilled or
deionized water sample. This test result is the reagent blank. Subtract the reagent
blank from all subsequent test results of unknown samples. It is necessary to
determine the reagent blank only when a new lot number of reagents is obtained.
SURFACTANTS SMART Spectro Test Procedures 8.13
Test P
rocedures