LaMotte SMART Spectro Spectrophotometer - Manual (Page 253)

PROCEDURE
Use universal sample holder.
1. Press and hold ON button until spectrophotometer turns on.
2. Scroll to and select PROGRAMMED TESTS.
3. Scroll to and select ALL TESTS (or another sequence containing
96 TANNIN) from TESTING MENU.
4. Scroll to and select 96 TANNIN from menu.
5. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with
sample.
6. Insert tube into chamber, close lid and select SCAN BLANK.
7. Remove tube from Spectro. Use the plain pipet (0352) to add 4 drops of
*Tannin Reagent #1 (7833). Cap and mix.
8. Use the 1.0 mL pipet (0354) to add 2.0 mL of *Tannin Reagent #2 (7834). Cap
and mix. Wait 30 minutes for full color development.
9. At end of 30 minute waiting period, mix, insert tube into chamber, close lid and
select SCAN SAMPLE. Record result.
10. Press OFF button to turn spectrophotometer off or press EXIT button to exit to
a previous menu or make another menu selection.
NOTES: For best possible results, a reagent blank should be determined to
account for any contribution to the test result by the reagent system. To determine
the reagent blank, follow the above test procedure to scan a distilled or deionized
water blank. Then follow the above procedure to perform the test on a distilled or
deionized water sample. This test result is the reagent blank. Subtract the reagent
blank from all subsequent test results of unknown samples. It is necessary to
determine the reagent blank only when a new lot number of reagents is obtained.
For the most accurate results, the sample and reagents should be at 20±2°C.
TANNIN SMART Spectro Test Procedures 2.11
Test P
rocedures