LaMotte SMART Spectro Spectrophotometer - Manual (Page 169)

PROCEDURE
Use universal sample holder
1. Press and hold ON button until spectrophotometer turns on.
2. Scroll to and select PROGRAMMED TESTS.
3. Select
ALL TESTS (or another sequence containing 48 H Per Shock) from
TESTING MENU.
4. Scroll to and select 48 H Per Shock from menu.
5. Use the pipet (0342) to add 5 drops of the sample water to a tube (0290).
6. Dilute to the 10 mL line with distilled or hydrogen peroxide-free water.
7. Insert the tube into chamber, close lid and select SCAN BLANK.
8. Remove the tube from spectrophotometer and add 4 drops of *Hydrogen
Peroxide Reagent #1 (6452). Cap and mix.
9. Add one *Hydrogen Peroxide LR Tablet (6454A). Crush tablet with Tablet
Crusher (0175). Cap and mix for 30 seconds. Solution will turn pink if hydrogen
peroxide is present. Wait 5 minutes for full color development.
10. At the end of 5 minute waiting period, mix, insert tube into chamber, close lid
and select SCAN SAMPLE. Record result.
11. Press OFF button to turn the spectrophotometer off or press EXIT button to
exit to a previous menu or make another menu selection.
NOTES: For best possible results, a blank should be determined to account for
any contribution to the test result by the reagent system. To determine the reagent
blank, follow the above test procedure to scan a distilled or deionized water blank.
Then follow the above procedure to perform the test on a distilled or deionized
water sample. This test result is the reagent blank. Subtract the reagent blank from
all subsequent test results of unknown samples. It is necessary to determine the
reagent blank only when a new lot number of reagents is obtained.
For the most accurate results, the sample and reagents should be at 25 ±4°C.
HYDROGEN PEROXIDE, Shock SMART Spectro Test Procedures 2.11
Test P
rocedures