LaMotte SMART Spectro Spectrophotometer User Manual

Page 13

SMART Spectro Operator’s Manual 2.11

Below is a list of suggested wavelength ranges for the color of the reacted

samples. Use these as a starting point.

Sample Color

Wavelength Range

Yellow

350-450

Yellow-Orange

450-490

Orange

490-510

Pink

510-570

Red

570-600

Green and Blue

600-750



CALIBRATION CURVES

The SMART Spectro contains precalibrated tests for the LaMotte reagent

systems. The fi rst step in using a non-LaMotte reagent system with the SMART

Spectro is to create a calibration curve for the reagent system. To create a

calibration curve, prepare standard solutions of the test factor and use the

reagent system to test the standard solutions with the SMART Spectro.
Plot the results (in ABS or %Transmittance) versus concentration to create

a calibration curve. The calibration curve may then be used to identify the

concentration of an unknown sample by testing the unknown, reading

Absorbance or %T, and fi nding the corresponding concentration from the curve.

The linear range of the reagent system can be determined and this information

can be used to input a User Test into the SMART Spectro (see EDIT USER

TESTS, page 35).

PROCEDURE
1. Prepare 5 or 6 standard solutions of the factor being tested. The

concentration of these standards should be evenly distributed throughout the

range of the reagent system, and should include a 0 ppm standard (distilled

water). For instance, the solutions could measure 0, 10%, 30%, 50%, 70%,

and 90% of the system’s maximum range.

2. Turn on the SMART Spectro. Select the appropriate %T/ABS wavelength from

the %T/ABS mode. Be sure to select the appropriate wavelength for the color

produced by the reagent system.

3. Use the unreacted 0 ppm standard to standardize the spectrophotometer by

using it to scan blank.

4. Following the individual reagent system instructions, react each standard

solution including 0 ppm. Record the reading and the standard solution

concentration on a chart. Readings can be recorded as percent

transmittance (%T) or absorbance (A).