LaMotte SMART Spectro Spectrophotometer - Manual (Page 163)

PROCEDURE
Use universal sample holder.
1. Press and hold ON button until spectrophotometer turns on.
2. Scroll to and select PROGRAMMED TESTS.
3. Scroll to and select ALL TESTS (or another sequence containing
45 Hydrazine) from TESTING MENU.
4. Scroll to and select 45 Hydrazine from menu.
5. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with
sample.
6. Insert tube into chamber, close lid and select SCAN BLANK.
7. Remove tube from Spectro. Use the 1 mL pipet (0354) to add 4 mL of
*Hydrazine Reagent A (4841). Cap and mix.
8. Use the 0.15 g spoon (0727) to add one measure of *Hydrazine Reagent B
Powder (4842). Cap and shake vigorously for 10 seconds. Wait 2 minutes for
maximum color development. An undissolved portion of Hydrazine Reagent B
may remain in bottom of tube without adversely affecting results.
9. At the end of the 2 minute waiting period, mix, insert tube into chamber, close
lid and select SCAN SAMPLE. Record result.
10. Press OFF button to turn spectrophotometer off or press EXIT button to exit to
a previous menu or make another menu selection.
NOTE: For best possible results, a reagent blank should be determined to account
for any contribution to the test result by the reagent system. To determine the
reagent blank, follow the above test procedure to scan a distilled or deionized
water blank. Then follow the above procedure to perform the test on a distilled or
deionized water sample. This test result is the reagent blank. Subtract the reagent
blank from all subsequent test results of unknown samples. It is necessary to
determine the reagent blank only when a new lot number of reagents is obtained.
HYDRAZINE SMART Spectro Test Procedures 2.11
Test P
rocedures