Eppendorf epMotion 5075 - Manual (Page 60)

Dispensing liquids
epMotion
® 5075
English (EN)
60
6.3.3 Liquid dispensing at the height of the vessel edge
In the software, the pipette tip can be positioned at a height of 3 mm – 4 mm below the edge of the vessel
using the
Dispense from top option. The height of the pipette tip does not change.
Use the option for:
• Quick dispensing
• Dispensing in vessels with small diameters, e.g., 384-well plates, capillaries
The tips remains in the upper area of the vessel and do not travel further into the vessel. This virtually
eliminates the possibility of the pipette tip becoming contaminated.
• Dispensing in plates with vastly different levels in the wells
6.3.4 Liquid aspiration from a filter plate
In the software, liquid can be suctioned from the supernatant of filter plates and filter vessels via the Elution
from filter option.
The Elution from filter option refers to the source vessel and almost fully aspirates liquid from the filter
plate. The pipette tip touches the filter. The piston movement in the dispensing tool for liquid aspiration
starts when the pipette tip moves in the vessel. Maximum stroke is used on each dispensing tool. This also
applies to dispensing.
When the liquid is being transported, air bubbles may be present at several locations in the pipette tip. The
size of the air bubble on the lower end of the pipette tip may vary.