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4 transporting and dispensing liquid, Transporting and dispensing liquid – Eppendorf epMotion 5075 User Manual

Page 55

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Dispensing liquids

epMotion

®

5075

English (EN)

55

6.1.4

Transporting and dispensing liquid

Abb. 6-3: Transporting and dispensing liquid

Fig. 6-3:

Transporting and dispensing liquid

6.1.4.1 Dispensing liquid

The liquid from the pipette tip will be dispensed in the free jet. The liquid will be dispensed into the vessel
3 mm – 4 mm above the surface of the liquid. The pipette tip moves upward during dispensing. The
distance from the pipette tip to the surface of the liquid remains constant.

For liquid type ProteinC, a distance of 5 mm from the pipette tip to the surface of the liquid is set in the
software. This setting takes into account the tendency of concentrated protein solutions to form foam.

6.1.4.2 Transporting liquids

The liquid is drawn into the pipette tip before transport. An area filled with air is created in the lower part of
the pipette tip. The liquid does not drip during transport.

A Dispensing liquid

B Retraction of liquid after liquid aspiration

~ 3 - 4 mm

~ 6 - 8 mm

A

B