Eppendorf epMotion 5075 - Manual (Page 58)

Dispensing liquids
epMotion
® 5075
English (EN)
58
6.2.3.1 Recommended mixing speeds
You can set mixing speeds of 0.2 mm/s – 110 mm/s.
6.3 Special features
6.3.1 Liquid aspiration from bottom of vessel
The pipette tip can also be positioned above the bottom of the vessel at the height of the bottom tolerance.
This prevents the pipette tip from changing its position during liquid aspiration.
The level in the vessels may not exceed the working volume. If the level exceeds the working volume, liquid
may overflow.
Liquid aspiration from the bottom of the vessel is recommended in the following cases:
• For vessels ≤0.5 mL, 96-well plates and 384-well plates
• For plates with various filling volumes in the wells
• For vessels with low filling volumes
Liquid aspiration from the bottom of the vessel can be activated in the software using the Aspirate from
bottom option (refer to the epMotion software operating manual). The bottom tolerance, which can be set in
the software, depends on the labware.
Abb. 6-4: Liquid displacement in vessels
Fig. 6-4: Liquid displacement in vessels
Dispensing tool Mixing speed of lower
volume range in mm/s Mixing speed of
medium volume range
in mm/s Mixing speed of upper
volume range in mm/s
TS 50 15 – 88 15 – 44 10 – 40
TM 50 15 – 88 15 – 44 10 – 40
TS 300 5 – 15 6 – 16 6 – 16
TM 300 2 – 11 2 – 11 2 – 11
TS 1000 4 – 15 4 – 15 4 – 15
TM 1000 4 – 15 4 – 15 4 – 15
A Vessel filled up to the working volume B Vessel with maximum immersed tip before
liquid aspiration
A B