Eppendorf epMotion 5075 - Manual (Page 59)

Dispensing liquids
epMotion ® 5075
English (EN)
59
6.3.2 Liquid aspiration from high vessels
Observe the following special features when using high vessels, e.g., 15 mL centrifuge tubes.
6.3.2.1 Liquid aspiration from bottom of vessel
Abb. 6-5: Large vessel with high liquid level
Fig. 6-5: Large vessel with high liquid level
If you use vessels > 3 mL with high levels, do not aspirate liquids from the bottom of the vessel. This will
result in the outside of the pipette tip being wetted, which increases the risk of contamination and distorts
the dispensing result. For very large vessels with high levels, the entire pipette tip and the tip cone of the
dispensing tool will be wetted.
6.3.2.2 High remaining volume
Abb. 6-6: Liquid aspiration from high vessels with 1000 μL, 50 μL and 300 μL pipette tips
Fig. 6-6: Liquid aspiration from high vessels with 1000 μL, 50 μL and 300 μL pipette tips
50 μL tips and 300 μL tips do not extend as far into the vessels as 1000 μL tips. In high vessels, 50 μL and
300 μL tips do not reach the bottom of the vessels. If you use 50 μL and 300 μL tips, the remaining volume
will be larger than with 1000 μL tips.
Work with the 1000 μL dispensing tool when you use high vessels.
If you use the 50 μL dispensing tool or the 300 μL dispensing tool, and would like to reduce
the remaining volume, select the lower vessels.