Techne PrimeQ User Manual
Page 9
9
3.3
Using Quansoft ................................................................................................................... 55
3.3.1
Home page ................................................................................................................ 55
3.3.2
Main screen ............................................................................................................... 56
3.3.3
Navigation bar ............................................................................................................ 56
3.3.4
Title bar functions ...................................................................................................... 57
3.3.5
Menu bar functions .................................................................................................... 58
3.3.6
Accessing the editors ................................................................................................. 59
3.4
Setting up an experiment ................................................................................................... 60
3.4.1
An Overview .............................................................................................................. 60
3.4.2
Creating a new experiment ........................................................................................ 61
3.4.3
Setting up a program ................................................................................................. 62
3.4.4
Setting up a plate layout ............................................................................................ 72
3.4.5
Defining the analysis method .................................................................................... 80
3.4.6
Saving an experiment to the library ........................................................................... 84
3.5
Running an experiment ...................................................................................................... 86
3.5.1
Starting the run .......................................................................................................... 86
3.5.2
Monitoring the run ...................................................................................................... 89
3.5.3
Stopping or pausing a run.......................................................................................... 91
3.6
LED intensity settings ......................................................................................................... 92
3.7
Results Editor ..................................................................................................................... 93
3.7.1
Post-run analysis main screen .................................................................................. 93
3.7.2
Viewing the results of a run ....................................................................................... 93
3.7.3
Editing a graph ........................................................................................................... 96
3.7.4
Changing the analysis parameters ............................................................................ 97
3.7.5
Log/Audit trail ............................................................................................................. 99
3.7.6
Report ........................................................................................................................ 99
3.8
Exporting and printing results ........................................................................................... 101
3.8.1
Exporting .................................................................................................................. 101
3.8.2
Printing ..................................................................................................................... 102
4
Data analysis .......................................................................................................................... 103
4.1
Introduction ....................................................................................................................... 105
4.1.1
Amplification curve ................................................................................................... 105
4.1.2
Thresholds ............................................................................................................... 105
4.1.3
Fit points .................................................................................................................. 106
4.1.4
First derivative maximum ......................................................................................... 107
4.1.5
Standard curve ........................................................................................................ 107
4.1.6
Dissociation curve .................................................................................................... 107
4.2
Choosing an analysis method .......................................................................................... 109
4.3
Analysis method: None .................................................................................................... 111
4.3.1
Viewing the results ................................................................................................... 111