Techne PrimeQ - Manual (Page 143)

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3.15.5 Quick guide to dissociation curve analysis
• In the Experiment or Results Editor Analysis Selection box, highlight the stage on which
Dissociation Curve is to be performed and click Edit.
• In the Analysis Wizard Selection box, choose Dissociation curve from the drop-down
menu and assign a use next to the appropriate dye name. Click Next.
• Background correction screen: Choose whether to smooth the data with a digital filter and
define where to position the cursors for correction. Click Next.
• Peak Detection: Choose to detect peaks automatically or manually. Set the number of
peaks and threshold filter for automatic detection or the bin threshold and threshold filter for
manual detection. Click
Next.
• Peak area: Check the box to have peak area calculated from the data. Click Next.
• Report options: Decide which data to display in the PrimeQ report. Click Next.
• Summary of analysis. Click Finish to return to the Experiment or Results Editor main page.
• The updated method appears in the Analysis Selection box in the Experiment or Results
Editor main screen.