Techne PrimeQ - Manual (Page 133)

133
3.14.8 Quick guide to quantification analysis
3.14.8.1 Quantification
1. In the Experiment or Results Editor Analysis Selection box, highlight the stage on which
quantification analysis is to be performed and click Edit.
2. In the Analysis Wizard Selection box, choose Quantification from the drop-down menu and
assign a use next to the appropriate dye name. Click Next.
3. Choose whether to correct the results with a PRD if a PRD was assigned in the dye usage box.
Click Next.
4. Select a baseline correction option. Click Next.
5. Choose a Cq calculation method. If choosing the fit points method, set the noise threshold,
crossing line and number of fit points. Click Next.
6. Report options: select which results should appear in the report. Click Next.
7. Summary of analysis. Click Finish to return to the Experiment or Results Editor main screen.
Relative quantification
1. Follow steps 1–3 as above but assign two reporters from the dye usage menu. Click Next.
2. Select a baseline correction option. Click Next.
3. Choose the Cq calculation method. Click Next.
4. Select the relative quantification method required. Choose from Relative Quantification or
Relative Cq and select which reporter is to be compared to which. Click Next.
5. Report options: select which results should appear in the PrimeQ report. Click Next.
6. Summary of analysis. Click Finish to return to the Experiment or Results Editor main screen.