11 technical specification – Techne PrimeQ User Manual
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1.11 Technical Specification
Thermal cycler
Block Format:
96 x 0.2ml well low-profile micro tube plate
Block Specification:
8 x Peltier block employing quad circuit technology
to enhance performance
Block Uniformity at 50ºC:
< ± 0.25ºC
Maximum Heating Ramp Rate:
2.2ºC/sec
Temperature Range:
4ºC to 98ºC
Sample Volume:
15µl to 50µl
Heated Lid:
Adjustable between 100ºC and 115ºC or off
Optical detection system
Excitation Source:
White LED
Detector:
Photon counting photo multiplier tube (PMT)
Multiplex Dye Detection:
Up to four dyes per reaction tube
User Selected Filters:
Maximum of four paired excitation/emission filter
cartridges suitable for use with currently available
dyes
Fluorescence Excitation Range:
470nm to 650nm
Fluorescence Detection Range:
500nm to 710nm
Dynamic Range:
At least nine orders of magnitude from starting
copy number.
Sensitivity:
1.0nM fluorescein in a 20µl sample
Single initial template copy detection*
Dimensions
Weight:
25kg
Size and Footprint:
420mm x 528mm x 518mm (W x H x D)
Power Supply:
100V to 230V, 50/60Hz
Input Power:
715W
IP code:
IP30
*
Assay dependent