Techne PrimeQ - Manual (Page 181)

181
PCR Efficiency An efficient PCR assay shows a doubling in the specific product
after every cycle:
Reaction efficiency E = 10
-1/slope
ideal value is 2
The efficiency is affected by the length, GC content and secondary
structure of the amplicon and sub-optimal reaction conditions.
PRD Passive Reference Dye: Internal reference dye to which the
reporter dye is normalized to correct for changes in volume and
block variations.
R
2
value Correlation coefficient which describes how well a line of Best Fit
describes the relationship between two sets of data. A value of
close to 1 defines good correlation whilst a value of 0 defines no
correlation.
REF Reference Gene. A gene expressed at an equivalent level in all the
samples in the real-time PCR experiment. Sometimes known as a
housekeeping gene.
Relative Quantification Used to analyse changes in expression in a sample relative to
another reference sample e.g. an untreated cell line.
Standard Sample of known concentration used to construct a standard curve
which is then used to extrapolate the quantity of an unknown
sample.