Techne PrimeQ - Manual (Page 10)

10
4.3.2 PrimeQ Report ......................................................................................................... 112
4.4 Passive reference dye (PRD) correction .......................................................................... 113
4.5 Analysis method: Baseline correction .............................................................................. 114
4.5.1 Assay requirements ................................................................................................. 114
4.5.2 Setup........................................................................................................................ 114
4.5.3 Viewing the results ................................................................................................... 116
4.5.4 PrimeQ Report ......................................................................................................... 118
4.5.5 Quick guide to baseline correction analysis ............................................................ 118
4.6 Analysis method: Quantification ....................................................................................... 119
4.6.1 Assay requirements ................................................................................................. 119
4.6.2 Setup........................................................................................................................ 119
4.6.3 Viewing the results ................................................................................................... 123
4.6.4 PrimeQ Report ......................................................................................................... 124
4.6.5 Using Cq values ....................................................................................................... 124
4.6.6 Comparing Cq values using a standard curve......................................................... 124
4.6.7 Comparing Cqs in relative quantification ................................................................. 128
4.6.8 Quick guide to quantification analysis ..................................................................... 133
4.7 Analysis method: Dissociation curve ................................................................................ 134
4.7.1 Assay requirements ................................................................................................. 135
4.7.2 Setup........................................................................................................................ 135
4.7.3 Viewing the results ................................................................................................... 141
4.7.4 PrimeQ Report ......................................................................................................... 142
4.7.5 Quick guide to dissociation curve analysis .............................................................. 143
4.8 Analysis method: Plus-minus scoring .............................................................................. 144
4.8.1 Assay requirements ................................................................................................. 144
4.8.2 Setup........................................................................................................................ 144
4.8.3 Viewing the results ................................................................................................... 146
4.8.4 PrimeQ Report ......................................................................................................... 148
4.8.5 Quick guide to plus-minus scoring analysis............................................................. 149
4.9 Analysis method: Allelic discrimination ............................................................................ 150
4.9.1 Assay requirements ................................................................................................. 150
4.9.2 Setup........................................................................................................................ 150
4.9.3 Viewing the results ................................................................................................... 152
4.9.4 PrimeQ Report ......................................................................................................... 155
4.9.5 Quick guide to allelic discrimination analysis........................................................... 156
4.10 Analysis method: Multi-read ......................................................................................... 157
4.10.1 Assay requirements ................................................................................................. 157
4.10.2 Setup........................................................................................................................ 157
4.10.3 Viewing the results ................................................................................................... 158
4.10.4 PrimeQ Report ......................................................................................................... 160