Luminex 100 IS Developer Workbench Guide Version 2.3 - Manual (Page 62)

Luminex 100 IS Developer Workbench Guide Version 2.3
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MAP Technology
56 PN 89-00002-00-084 Rev. B
Oligonucleotide Coupling Protocol
Introduction
Use these protocols as a general starting point for developing assays.
Optimize all assays for your reagents in your specific application.
Updates or additions to these protocols are posted on the Luminex
website at
http://luminexcorp.custhelp.com. At the main page, select
a subject or perform a search for the desired information.
You obtain the best results by starting with these guidelines and
modifying them for your specific needs.
Equipment
• Vortex
• Sonicator bath
• Micropipetters: (1 µL - 1000 µL)
• Microcentrifuge
• Microfuge tubes (USA Scientific)
• Timer
• Analytical balance
Materials
• xMAP carboxylated microspheres—LIMIT EXPOSURE TO
LIGHT!
• Amino-substituted oligonucleotide (See Technical note 1)
• Microcentrifuge tubes: 1.5 mL, polypropylene (see the following
technical notes)
• Pipetter tips: 1 µL - 1000 µL
• Pierce EDC:
1-Ethyl-3- (3-dimethylaminopropyl) carbodiimide hydrochloride
• MES:
0.1 M 2- (N-morpholino) ethane sulfonic acid pH 4.5, filter,
sterilize, and store at 4°C.
• Tween-20 (0.02% v/v)
• SDS:
Sodium Dodecyl Sulfate (0.1% w/v)
• dH
2
O
• TE Buffer (10mM Tris, 1mM EDTA, pH 8.0)
Preparation
1. Allow all reagents to warm to room temperature.
2. Resuspend the amine-substituted oligonucleotide to 1.0 mM in
sterile, deionized water.