Luminex 100 IS Developer Workbench Guide Version 2.3 - Manual (Page 26)

Luminex 100 IS Developer Workbench Guide Version 2.3
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MAP Technology
20 PN 89-00002-00-084 Rev. B
17. These commands become part of the template and are always
performed in the order specified. Each well command is
automatically assigned to the next available well. Laboratory
personnel can select the starting location on the plate, but they
can not change these commands or the order in which they are
performed. Remember that if you have more than one command
that involves the reservoir, those commands must use the same
solution.
Table 3 lists available template commands.
18. After you enter all the information, click Save to save the
template, then click Close to exit.
Table 3: Available Commands
Command Definition
Acquire Acquires an unknown sample.
Acquire
Background Acquires a background sample from a well. The
background results are automatically subtracted
from standards, controls, and samples. If you are
running more than one background well, the
software will subtract the average of the
background samples.
Acquire Control Acquires the specified control from a well. If a
specified control is selected more than once, the
software calculates the results for each well
independently. It then reports an average of the
control samples.
Acquire Standard Acquires the specified standard from a well. If a
specified standard is selected more than once, the
software calculates the results for each well
independently. It then reports an average of the
two samples.
Acquire Test
Specimen Acquires a sample reading from a well. If Acquire
Test Specimen is selected more than once, the
software calculates the results for each well
independently. However, samples having identical
Sample IDs are treated as replicates reporting an
average of the individual samples.
Backflush Backflushes the system by reversing the flow of
sheath fluid through the cuvette.
Calibrate CAL1 Acquires calibration beads from a well to calibrate
the Classification and DD channels.
Calibrate CAL2 Acquires calibration beads from a well to calibrate
the Reporter channel.
Note: You can return and edit
any unused templates using the
Edit Template feature.