Luminex 100 IS Developer Workbench Guide Version 2.3 - Manual (Page 55)

PN 89-00002-00-084 Rev. B 49
xMAP Protocols
Updates or additions to these protocols are posted on the Luminex
Tech Support website at
http://luminexcorp.custhelp.com. At the
main menu, select a subject or perform a search for the desired
information.
The protocols in this appendix are presented in the following order:
‰ Protein Coupling Protocol
‰ Oligonucleotide Coupling Protocol
‰ LumAvidin™ Binding Protocol
xMAP Microsphere
Handling
xMAP Microsphere
Dispersion
Luminex xMAP microspheres settle and aggregate if left
undisturbed. You should always ensure that they are evenly
distributed before dispensing. Gentle vortexing and sonication are an
effective method of mixing for most coated xMAP microsphere
preparations. After dispersion, xMAP microspheres generally remain
in suspension for about one hour. To maintain bead concentration
within the stock xMAP microsphere volumes (1 mL, 4 mL, or 16
mL), we suggest that you follow these guidelines.
For complete resuspension of the 1 mL stock vials, centrifuge briefly
to pool the entire volume, then sonicate and vortex. Failure to do so
often results in decreased amounts of xMAP microspheres recovered
in future sample aliquots.
For 4 mL and 16 mL vials, place on a rotator for a minimum of 15
minutes for complete resuspension. After using approximately 250
µL from the vial, you can reduce the rotator time to 1 to 2 minutes.
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