Luminex 100 IS Developer Workbench Guide Version 2.3 - Manual (Page 11)

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MAP Technology
General Guidelines
PN 89-00002-00-084 Rev. B 5
The volume restrictions that the amount expelled back places on the
assay design can be expounded by the following formula:
Sample Timeout
Sample Timeout is the amount of time in seconds that the Luminex
100 analyzer spends to read the sample. It uses this time to detect the
specified number of events.
If sufficient events are not collected in the specified time, the
Luminex 100 IS system logs an error, flags the sample, and goes on
to the next well. If you don’t want to use the timeout property, set the
sample timeout to zero.
Sample Dilution
Dilute serum samples at least 1:5 with reagents as part of assay setup
or as a final dilution step.
Reagents
Formulated reagents must be free of particulates other than xMAP
microspheres.
Kit Instructions
Include in your kit instructions:
• Dilution and dilution reagent
• Preparation steps, such as sample probe height adjustments to
accommodate the selected plate type
• Luminex XYP instrument heater temperature setting
• Information about assay controls and standard values for each
test in the assay as product lot information
• Template name and version number in the kit instructions
• Provide template on a 3½” diskette or CD
[“Total well volume” (µL ) – “Sample uptake volume”(µL)] + 160(µL)
Where:
Total well volume The starting sample volume of a well
before the unit samples for acquisition.
Sample uptake
volume The uptake volume for acquisition
(program this in the template as sample
vol.)
160µL The volume expelled back as stated in
the above paragraph.
Maximum well
volume plate The maximum volume capacity of the
wells in a user’s selected 96 well
microtiter plate.