Luminex 100 IS Developer Workbench Guide Version 2.3 - Manual (Page 12)

Luminex 100 IS Developer Workbench Guide Version 2.3
x
MAP Technology
6 PN 89-00002-00-084 Rev. B
Gating
Determine the gating on the doublet discriminator channel for the
assay during development. The numeric values appear on the left
side of the histogram. Use the numerical gate position, as determined
in development, to set the gate location in the template.
Gating information may change with a new lot of xMAP
microspheres. Each time you receive a new lot of xMAP
microspheres, re-evaluate them with the current templates. If gating
information changes, create a new template identical to the current
template, but with a new version number and new gating
information.
Plates
For heated assays, use:
CoStar ® Thermowell ® 96-well plate, thin-wall polycarbonate,
model P
For nonheated assays:
Select a 96-well plate with an overall height no greater than 0.75
inches (19 mm).
Instrument Calibrators
and Controls
The Luminex 100 IS system requires calibration:
• at initial system installation.
• at least once a month.
• if the “dCal temperature” (difference of current temperature and
the last calibrated—between the yellow arrows on the DD Temp
gauge) changes by more than ±3°C.
• if the system is moved.
• if parts are replaced.
• if sample acquisition is problematic.
For further information on performing analyzer calibrations, see the
Luminex IS Software Manual for Version 2.3.
Note: Gate positions are
dependent upon buffer
composition. Any changes made
to the buffer composition in your
assay may result in a different
optimal gate location.
Note: When using uncovered
plates, use black opaque plates,
if possible, to reduce
photobleaching.