Metrohm 833 IC Liquid Handling Unit - Manual (Page 77)

8.2 Assembling the ultrafiltration cell
833 IC Liquid Handling Unit / Instructions for Use 8.833.1003
69
8.2 Assembling the ultrafiltration cell
This section describes the initial assembly of the ultrafiltration cell.
Before the ultrafiltration cell is used a filtration membrane must be
inserted. Proceed as follows (see Fig. 22):
1 Prepare ultrafiltration cell
• Remove ultrafiltration cell 66 (6.2729.100) from the packaging
and remove the three screwed-in 6.2744.060 Blank stoppers .
• Use the 6.2621.070 Allen key to loosen the 5 screws 70
completely. Separate lower part 69 from upper part 67 and
remove sealing ring 68.
2 Clean ultrafiltration cell
• Thoroughly the clean sealing ring 68, upper part 67 and
lower part 69 of the ultrafiltration cell with ultrapure water and
dry with N
2
or a lint-free tissue.
Only ultrapure water or ethanol should be used for cleaning the
dialysis cell, other organic solvents (e.g. acetone) will damage the
Plexiglas cell!
3 Prepare filtration membrane
• Use the 6.2831.010 Tweezers to remove a new filtration
membrane 72 (e.g. 6.2714.020) from the packaging and
immerse in a Petri dish filled with ultrapure water for approx. 2
min until the membrane is thoroughly soaked with water.
4 Insert filtration membrane
• Place upper part 67 on a paper tissue with its inner side
uppermost.
• Place sealing ring 68 in the recess provided for it in upper
part 67.
• Use the 6.2831.010 Tweezers to place the wet filtration
membrane 72 (e.g. 6.2714.010) inside sealing ring 68 on
upper part 67.
Make sure that the filtration membrane soaked with water does not dry
out before insertion as otherwise it cannot be used !
5 Close ultrafiltration cell
• Place lower part 69 on upper part 67 so that the two parts are
properly aligned with each other.
• Use the 6.2621.070 Allen key to screw in the 5 screws 70
completely and tighten them up well.