beautypg.com

Metrohm 833 IC Liquid Handling Unit User Manual

Page 54

background image

6 833 IC Liquid Handling Sample Preparation Unit

833 IC Liquid Handling Unit / Instructions for Use 8.833.1003

46

4 Connect sample preparation module to valve A

(Sample Preparation connection 1)

Connect outlet capillary 41 (marked with “Sample out”) at

sample preparation module connection 34 (see Fig. 13) to

connect “1” of injection valve A using a 6.2744.010

compression fitting.

5 Mount pump tubing on 833 Liquid Handling Unit

Mount the two lengths of 6.1826.010 Pump tubing (white-

white stopper) as described in Section 3.2, see Fig. 7.

6 Sample preparation module connection 2:

Regenerant

Pull inlet capillary 37 (marked with “Regenerant”)at sample

preparation module connection 34 (see Fig. 13) through one

of the openings 18 on the rear panel of the 820 IC Separation

Center.

Attach inlet capillary 37 (23 in Fig. 7) to filter unit PEEK 22

(6.2821.120) of the first pump tubing using a compression

fitting 17 (6.2744.010), see Fig. 7.

Mount aspiration tubing: cut a piece of PTFE tubing 16

(6.1803.020) to the required length.

Attach a PEEK compression fitting 17 (6.2744.010) to one

end of the PTFE tubing 16 and screw this on to coupling 18,

see Fig. 7.

Immerse the other end of the aspiration tubing in a container

with regeneration solution (normally 50 mmol/L perchloric

acid) and fix it in position.

Pull outlet capillary 38 (marked with “Waste”) at sample

preparation module though one of the openings 18 of the

rear panel of the 820 IC Separation Center.

Lead outlet capillary 38 into a sufficiently large waste bottle

and fix it in position.

7 Sample preparation module connection 3: H2O

Pull inlet capillary 40 (marked with “H

2

O”) at sample

preparation module connection 34 (see Fig. 13) through one

of the openings 18 on the rear panel of the 820 IC Separation

Center.

Attach inlet capillary 40 (23 in Fig. 7) to the filter unit PEEK 22

(6.2821.120) of the second pump tubing using a PEEK

compression fitting 17 (6.2744.010), see Fig. 7.

Mount aspiration tubing: cut a piece of PTFE tubing 16

(6.1803.020) to the required length.

Attach a PEEK compression fitting 17 (6.2744.010) to one

end of the PTFE tubing 16 and screw this on to coupling 18,

see Fig. 7.