Metrohm 833 IC Liquid Handling Unit - Manual (Page 6)

Table of contents
833 IC Liquid Handling Unit / Instructions for Use 8.833.1003
II
6.2 Connection to modular IC system for cation separation ........................43
6.2.2 Settings in IC Net.............................................................................................. 47
6.3 Handling the sample preparation module................................................49
7 833 IC Liquid Handling Dialysis Unit ....................... 50
7.1 Flow diagram for dialysis..........................................................................51
7.2 Electrical connection.................................................................................52
7.2.1 Dialysis without suppression............................................................................ 52
7.2.2 Dialysis with suppression................................................................................. 53
7.3 Settings in IC Net.......................................................................................54
7.3.1 Dialysis without suppression............................................................................ 54
7.3.2 Dialysis with suppression................................................................................. 55
7.4 Assembling the dialysis cell .....................................................................56
7.5 Making the capillary connections.............................................................57
7.5.1 Preparing the 820 IC Separation Center.......................................................... 57
7.5.2 Connection of the dialysis cell ......................................................................... 58
7.6 Optimizing the dialysis..............................................................................63
7.6.1 Determining the rinsing time ............................................................................ 63
7.6.2 Determining the transfer time........................................................................... 63
7.6.3 Determining the dialysis time........................................................................... 64
7.7 Dialysis procedure ....................................................................................66
8 833 IC Liquid Handling Ultrafiltration Unit.............. 68
8.2 Assembling the ultrafiltration cell.............................................................69
8.3 Connect filtration cell ................................................................................70
8.4 Filtration .....................................................................................................74
8.4.1 Selection of possible sample types ................................................................. 74
8.4.2 Filter working life............................................................................................... 74
8.4.3 Filter membrane selection................................................................................ 75
9 Troubleshooting - maintenance............................... 76
9.1 Faults and their remedies .........................................................................76
9.2 Care and maintenance ..............................................................................79
9.2.1 Instrument care ................................................................................................ 79
9.2.2 Maintenance by Metrohm service.................................................................... 79
9.2.3 Shut down ........................................................................................................ 79
9.2.4 Replacing the pump tubing ............................................................................. 80
9.2.5 Changing the filter ............................................................................................ 81
9.2.6 Suppressor module / Sample preparation module......................................... 82
9.2.7 Dialysis cell....................................................................................................... 88
9.2.8 Ultrafiltration cell............................................................................................... 90
10 Appendix ................................................................... 91
10.1 Technical data............................................................................................91
10.1.1 Basic instrument: 833 IC Liquid Handling Unit 1.833.0010............................. 91
10.1.2 6.2729.100 Dialysis cell.................................................................................... 94
10.1.3 6.2729.110 Ultrafiltration cell............................................................................ 94
10.2 Standard equipment ..................................................................................95
10.2.1 833 IC Liquid Handling Pump Unit .................................................................. 95
10.2.2 833 IC Liquid Handling Suppressor Unit ......................................................... 96
10.2.3 833 IC Liquid Handling Sample Preparation Unit............................................ 97
10.2.4 833 IC Liquid Handling Dialysis Unit ............................................................... 98
10.2.5 833 IC Liquid Handling Ultrafiltration Unit...................................................... 100